Titan is much more potent than Venom, requiring only a small amount for a Bane-like reaction. Earlier versions of Titan often killed the test subjects due to the massive shock to the body causing it to fail and die from the compound soon after receiving it.
The treatment has a price, as the sudden release of endorphin and adrenaline, along with the extreme pain of the transformation, results in the recipient going into a crazed, psychotic state. The subject undergoes not only a size change but grotesque facial and muscular disfigurement, even causing their spinal columns to become exposed.
Only Bane (due to his extreme intelligence and will, and his previous usage of Venom and the TN-1), Poison Ivy (due to her natural immunity to toxins), and the Joker (because of his high intelligence and his psychopathy) retained their personalities (at least, partially) and some of their intelligence.
In the case of the Joker, he retained his normal personality after injection, as he was already insane, actually enjoying the painful transformation, which in his case caused his exposed spinal column to resemble a set of dorsal fins. Batman, having taken a Titan-filled dart meant for the captive Jim Gordon, chose to resist the change and use the antidote on himself, much to Joker's chagrin. It is possible the drug reduced even Joker's intelligence, for while the fight was a ferocious one, Batman had no trouble finding a strategy to bring Joker down. Whatever his overall health, he did not look well when shown in withdrawal during the game's closing scenes, nor did his formerly enhanced henchmen.
Before Arkham Asylum incident Edit
TITAN was originally conceived by Dr. Penelope Young as a way for weaker patients to survive more strenuous treatments; additionally, it's believed that Scarecrow aided in it's creation. However, Young's source of funds Jack White, who was in reality the Joker, intended to use the compound to create an army "of a thousand Banes".
Bane was secretly brought to Arkham Asylum (arranged by the Joker). Joker's plan was to have Dr. Penelope Young study him and the effects of Venom on the human body, so that she could develop TITAN, a more powerful variant of Venom. When discussing the Titan Project in public, Bane's involvement was kept secret, and he was referred to only as "Patient X". Officially, Bane was listed as an escapee from Blackgate Penitentiary to cover-up his transfer (Batman and the GCPD were led to believe that he had escaped Blackgate). During this time, Bane was forced against his will to be a human guinea pig for the Titan Project.
One of their first test subjects was Katie Burke, the daughter of millionaire Edward Burke. She was diagnosed with a deadly disease, and her father started looking for a cure. He found a doctor that offered her help, Harleen Quinzel. She introduced Edward to a friend of his, Jack (Joker). As Joker persuaded Edward to build an amusement park on an oil rig, Harley pretented to do tests, and claimed that the only way Katie could be cured was through the use of TITAN, the experimental drug. Ready to try anything to cure his daughter, Edward agreed with the decision. As expected, Katie died painfully, driving her father to commit suicide, allowing Joker to take control of the amusement park.
Arkham Asylum IncidentEdit
While Young discovers the identity of her benefactor and attempts to kill the project, research had already progressed into the production phase. Intent on obtaining the formula, Joker formulated the Arkham incident partially to obtain the Titan and create his "monster army". Joker succeeded in using the formula on several of his henchmen and Arkham Guards transforming them into Bane like creatures as well as on Poison Ivy enhancing her powers and nearly destroying the island with killer plants and finally himself. He also used Titan on Bane against his will to have him fight against Batman and to test out the Titan's effects.
It is revealed by Poison Ivy that there is a counter-compound/antidote to Titan, which originates from unique plant spores found in Killer Croc's Lair. Batman was able to obtain some spores and liquefy them to make an antidote, unfortunately Ivy's plants destroyed the Bat-Computer that was mass producing the antidote. Luckily enough, the Titan eventually wore off on its own, returning the affected individuals to normal, including the Joker who used it on himself as a last resort to kill Batman. Though the original Titan compound and formula was destroyed, one crate containing mass produced Titan was left floating in the water.
After Arkham Asylum IncidentEdit
Following the incident at Arkham Asylum, it is revealed that the Titan mixture has made its way into the Gotham underground. Crates of the compound floated into the docks and Two-Face bought it hoping for quick cash. Little did he realize the mistake of having Brother-Sister team Terry and Tracy Trask take them into his cellar. Upon discovering what it was, the siblings started using it and turned the cellar into a personal training ground.
After slaughtering Two-Face's gang and putting the man himself into several casts, T&T started making plans to take over the city under the employment of Hugo Strange. Unfortunately for them, Batman arrived in time to save the city, but before he could trace them back to Hugo Strange, they committed suicide via high explosives planted inside their bodies, killing hundreds and blowing up Gotham City Hall.
Additionally, the Joker is still suffering from the side-effects of his Titan overdose, which has mixed with the toxins in his blood and mutated into a extremely toxic poison with an estimated six months to live. Joker is planning to use the Titan again having had a doctor, which he promptly sent into a incinerator, isolate the Titan from his blood. In addition, the Joker had some of the Titan formula that was isolated from his blood injected into one of his henchman, and then sent him after Bane.
After the henchman managed to place Bane at his mercy, however, the henchman started to suffer from cardiac arrest, resulting in Bane having to mercy kill the henchman. Due to this experience, Bane also vowed to destroy the source of all Titan by any means necessary. This led Bane to Penguin's latest gang initiation where he demanded that Penguin hand over all of his Titan immediately.
While Bane was able to defeat Penguin's Titan Henchman, he was forced to retreat by Solomon Grundy.
Arkham City IncidentEdit
After his encounter with the Titan-infused thug, Bane launched a crusade against the existence of the Titan drug, devoting himself to the eradication of the compound. However, Bane soon decided that it was best to use the Titan to enhance his own strength instead. Realizing that he couldn't do it alone, he signaled Batman with a flare and lured him into the abandoned Krank Co. Toy Factory in the flooded section of Arkham City. There he alerted Batman to the twelve Titan containers remaining within Arkham City.
Forming a partnership, Batman destroyed the six containers dotted around the south of Arkham City while Bane took the northern six. Returning to their meeting, however, the pair are ambushed by TYGER Guards and Bane reveals his intent to hoard the Titan to use for his own purposes. Anticipating his betrayal, Batman locks him up and uses Explosive Gel to destroy the Titan Bane had gathered; finally ridding Gotham of the Titan Compound.
Meanwhile, the Joker; dying from his previous exposure to the compound during the Arkham Asylum incident, uses his infected blood to poison stores of medical blood across Gotham, and infecting Batman himself, using this as a bargaining chip to force Batman to find a cure for the poison before it kills them as well as him. Prompted to contact Mister Freeze for research into a cure, Freeze reveals that he had made an unstable formula that could theoretically work.
Noting that all the formula needed was a specific enzyme that could be found in the blood of Ra's al Ghul (due to his constant usage of Lazarus Pits), Batman managed to locate the League of Assassins stronghold underneath Arkham City in the old Wonder City, and obtained a sample of Ra's blood. Returning the sample to Freeze, Harley Quinn managed to steal the developed cure as Freeze was attempting to use it as a bargaining chip to force Batman to find his wife, Nora Fries.
The cure was in turn stolen from Harley by Talia al Ghul which prompted Joker to kill her for it, and the cure then wound up in the hands of Clayface. When Batman defeated Clayface and took the cure, he managed to cure himself just before Joker, believing that Batman would never give him the cure, stabbed him in the arm in an attempt to get at it. This prompted Batman to drop the vial, where it smashed on the floor.
Unable to cure himself, and laughing at the irony that Batman would have given him the cure had he not attempted to steal it, the Joker succumbed to the Titan poisoning, and died. Batman apparently missed some of the Titan, as Harley Quinn used it on one of her thugs two weeks following Batman and Bane's alliance.
Between Arkham City and Arkham Knight Edit
Though cured of the fatal condition the Titan-infected blood had caused, Batman could sense something was still up the now-deceased Joker's sleeve. He soon found four transfusion bags of Joker's blood were unaccounted for, resulting in four people being infected with it. Christina Bell, Albert King, Johnny Charisma and Henry Adams were detained by Batman and studied; as a result of the infected blood gestating in Bell, King and Charisma for too long, there were beginning to look and act like Joker, although with a specific aspect of the Clown Prince of Crime's personality. Only Henry seemed immune to this. Batman became worried that he may end up becoming "Joker-ized" himself, keeping a cell ready in case he ever succumbed.
Batman tested Henry constantly to see what was allowing him to resist Joker's influence.
Arkham Knight Incident Edit
In truth, Henry was infected, but was able to hide the effects. He made contact with Harley Quinn prior to Batman incarcerating him, and plotted with her to free the new Jokers, with the hidden intention of killing them to purify the gene pool. Scarecrow's fear toxin only accelerated the process in Batman, causing him to see the Joker talking to him whenever he was facing a trying situation. After Henry killed Bell, Brown and King, he committed suicide after seeing Batman's eyes turn green as a sign the Joker was inside him and would make him the best candidate to replace their progenitor.
Batman later was injected with 10 vials of Scarecrow's improved fear toxin, causing the Joker to surface. Thus this begins a mental battle between Batman and the Joker, showing him the only fear the Clown Prince of Crime had - being forgotten, even by Harley. Confronting the Joker in a version of Arkham, Batman managed to finally install the fear in Joker that he never could, by locking him away in a dark corner of his mind.
- Titan seems to have different effects on metahumans. When Poison Ivy was injected with Titan, her plants got stronger and larger while Ivy herself remained largely unchanged, save for her eyes obtaining a green glow. Her chlorokinetic abilities also seemed to become enhanced.
- TITAN-poisoning seems to be unavoidable if an overdose is taken. In already altered humans, the remnants of the TITAN would react badly with the chemicals already in their blood.
- When a normal henchman is injected with the Titan formula, the henchman will mutate, and will become insane. However, when the Joker injected himself with Titan, even though he had mutated, he kept his personality. This could very well be because he is already insane.
- When the Joker injected himself with Titan and became a mutated monster, he seems to have gained a mohawk instead of his normal hair style, which is very similar to the Joker taking Bane's Venom in the cartoon series The Batman. This episode of the cartoon could very well have been an inspiration for this appearance of the Titan-enhanced Joker.
- Following the credits of Batman: Arkham Asylum, three alternate endings show either Bane, Scarecrow, or Killer Croc grabbing the wayward Titan crate. The ending shown depends on how many times the particular character has killed the Batman.
- In Batman: Arkham City, depending on what point in the game the player chooses to meet Bane, Batman will either find out about the compound's existence within the city from Bane or already know about the compound from a run-in with the Penguin.
- There was a long-run rumor before Arkham City was released that says Penguin would use the Titan in the game, but it was proven to be fake. However, the Penguin does have a single Titan enhanced henchmen.
- In Batman Arkham Origins, Bane injects himself with TN-1, a super version of his Venom that gives highly increased powers at the expense of memory loss. This potentially provides an explanation for why the super-villain was as he was in Asylum and City.