Protocol 10 was devised by Hugo Strange and Ra's al Ghul to wipe out the criminal element in Arkham City, along with anyone else that could pose a threat to them by revealing the secrets about the prison - Political Prisoners. Presented to the Gotham City Council as a last resort if the inmates gained access to weapons and were planning a mass breakout, that procedure would grant the TYGER Guards full permission to exterminate the inmates with missile strikes via helicopter.
As extra ensurance that major threats like the super criminals were eliminated by Protocol 10, Strange had the Observation Deck of Wonder Tower modified to hold several missile launchers. These missile would be more powerful that the ones launched by the helicopters, capable of taking out a large portion of a building.
After he incarcerated Bruce Wayne, Strange announced that Protocol 10 would commence within 10 hours. After he took an encryption chip from a TYGER Guard, Batman was immediately informed of the impending deadline as he progressed through the prison. He event tossed his old pawn Quincy Sharp into the prison, a feat that amazed the inmates as it should have been impossible to get the mayor arrested. Ensuring that his Political Prisoners would die, Strange hired the assassin Deadshot, whom he likely kept in the dark about Protocol 10.
Strange went to City Hall, were he lied about the inmates gaining control of weapons despite his best efforts to prevent them from gaining weapons; in truth he had given both the Penguin and the Joker various firearms to ensure that his actions of attacking the prison were justified. It was likely that Strange had kept the Mayor's arrest a secret at the time, just to ensure he could get what he wanted. Thought City Hall was disappointed in him, they gave him permission anyway, unaware that Strange had imprisoned the mayor in Arkham City.
Upon learning that Batman had broken into the Joker's lair, Strange made it his first target. Batman and the Joker had survived the first attack (as Joker had fled earlier and Catwoman helped Batman out of the rubble). Once in full force, goons belonging to the Joker and the Penguin hid in the Sewer and Subway tunnels. Others hid by the walls of the prison. Batman downloaded the Master Control Code, infiltrated Wonder Tower and shut down Protocol 10. The only major damage suffered during the attack was suffered by the hideouts of the Joker, the Penguin and Two-Face, while the rest of Arkham City remained intact. It also appeared that the henchmen of Scarecrow were active and killed during the attack.
In Batman: Arkham Knight, it was revealed that many former Arkham City inmates had sued Gotham City Hall for giving Strange the permission to go ahead with Protocol 10 which put all their lives in danger. Those who won their court cases received a large cash payout and were even released from prison. It's possible that supervillains like the Mad Hatter and Deadshot
- After Protocol 10 is done, the TYGER guads no longer patrol Arkham City. Likely they were arrested, or left catatonic without their master Hugo Strange to direct them.
- The Politcal Prisoners managed to survive by hiding under a bridge.
- Strange mentions that Phase Two of Protocol 10 would be repeating Arkham City to wipe out the enemies of Superman and Flash.