Arkham Origins Blackgate Incident
Twenty Joker Teeth were scattered all around the Administration portion of Blackgate Prison. Those teeth were armed with explosives, and blew up if they were either destroyed, or if Batman walked too close to them. If the player destroyed them all, they were awarded with a trophy.
A Matter of Family Incident
Arkham Asylum Incident
Joker used the teeth to distract Batman through the story. It also served as one of Riddler's Challenges. It was also one of Joker's gadgets in the Predator Challenges in the DLC "Play as The Joker", but much like the ones found in the Blackgate Incident, they were equipped with explosives.
Walking versions of the teeth were also used by the Joker while fighting Batman upon injecting himself with Titan, and similar to the Predator Challenges as aforementioned, they also were equipped with explosives. When they get close to Batman, they start jumping up and down as well as making comical honking noises before exploding.
Arkham City Incident
Again, Joker used the teeth to distract Batman, but instead of the teeth being spread across the whole map, they were only in a few parts of it, like the Sewers and the Subway.
- They also appeared in the game, Injustice: Gods Among Us as one of Joker's attacks.