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Cold Cold Incident
Some of Penguin's Henchmen used Freeze Guns to try to freeze Batman in ice. Others used them in various crimes, including a heist that ended with their use of a cryogun somehow setting the building that they were in, on fire. Mr. Freeze used the Freeze Gun to try to stop Batman from rescuing Ferris Boyle at GothCorp.
Arkham City Incident
The Freeze Gun first appeared with Penguin, who took the gun and suit from Mr. Freeze. After he rescued Freeze from the War Room at the Museum, Batman went after Freeze's Suit to get a chip that deactivated the Freeze Gun. After he got it, Batman went after Penguin, turned off the Freeze Gun, and defeated him.
After he returned from the Demon Trials with Ra's al Ghul's blood, Batman was confronted by Freeze, who uses his Freeze Gun to try to kill him. Freeze was defeated, the Freeze Gun didn't appear again.
Before Arkham Knight Incident
Arkham Knight Incident
The Freeze Gun remained in the Evidence Room at the GCPD Lockup. Freeze never stole it back from the GCPD, as he's upgraded his Armor to have a wrist gun.
- In the final battle with Freeze during the Cold, Cold Heart, Freeze demonstrated that he could use the first version of the Freeze Gun as a method of propulsion to climb onto platforms.
- It was assumed that all of the cryo-guns that Freeze created for Penguin's Henchmen were destroyed after the New Year's Eve Incident.